Context
In vivo lipids dynamics studies is mainly realized using rodent models. Parameters evaluation of is sometimes difficult due to the size of such models. An obese insulin-resistant dog model (OBEIR)conform to response observed in Human has been developed. However this canine model two fundamental differences compared to Human :
- cholesterol is mainly transported by HDLs;
- absence of CETP , “shunt enzyme”, who played a major role in Human.
Aims
- Get validation limits of the current OBEIR model;
- wider evaluation of response conformity to pharmacological, pathological and environmental stimuli;
- refine model;
- derive model to match specific needs;
- explore other possible big size model (cat, minipig, horse);
Techniques
- Chromatography FPLC, HPLC, GC;
- Lipoprotein kinetics;
- Physical activity : GPS tracking, effort intensity;
- Biological parameters : chemical assessments, RIA, IP, enzymatic activity, body composition, energy expenditure;
Study axes
Main results
- dog breeds susceptibility with high LDLs levels in pathological conditions;
- OBEIR model : support workout mimicking regular physical activity;
- OBEIR model : response to drug treatment aiming pharmacological induced hypothyroidism;
- encouraging preliminary results with candidate species (horse, cat, minipig…).
Conclusion
- Development axis shows encouraging results despite in situ difficulties;
- Multiple adaptations made it tremendously convenient;
- Wide margin to reveal potential of methodologies considered for this project.
