Animal models : obesity, diabetes and IR

Animal models : obesity, diabetes and IR

Context

In vivo lipids dynamics studies is mainly realized using rodent models. Parameters evaluation of is sometimes difficult due to the size of such models. An obese insulin-resistant dog model (OBEIR)conform to response observed in Human has been developed. However this canine model two fundamental differences compared to Human :

  • cholesterol is mainly transported by HDLs;
  • absence of CETP , “shunt enzyme”, who played a major role in Human.

Aims

  • Get validation limits of the current OBEIR model;
  • wider evaluation of response conformity to pharmacological, pathological and environmental stimuli;
  • refine model;
  • derive model to match specific needs;
  • explore other possible big size model (cat, minipig, horse);

Techniques

  • Chromatography FPLC, HPLC, GC;
  • Lipoprotein kinetics;
  • Physical activity : GPS tracking, effort intensity;
  • Biological parameters : chemical assessments, RIA, IP, enzymatic activity, body composition, energy expenditure;

Study axes

Main results

  • dog breeds susceptibility with high LDLs levels in pathological conditions;
  • OBEIR model : support workout mimicking regular physical activity;
  • OBEIR model : response to drug treatment aiming pharmacological induced hypothyroidism;
  • encouraging preliminary results with candidate species (horse, cat, minipig…).

Conclusion

  1. Development axis shows encouraging results despite in situ difficulties;
  2. Multiple adaptations made it tremendously convenient;
  3. Wide margin to reveal potential of methodologies considered for this project.
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WPdrx
Physiologist Bioinformatician
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